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polymerase chain reaction

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polymerase chain reaction - (Abbreviation: PCR). A widespread molecular biology procedure that allows the production of multiple copies (amplification) of a specific DNA sequence, provided that the base pair sequence of each end of the target is known. It involves multiple cycles of DNA denaturation, primer annealing, and strand extension, and requires a thermostable DNA polymerase, deoxyribonucleotides, and specific oligonucleotides (primers). [FAO]

News archive for polymerase chain reaction.

American College of Gastroenterology Issues Guidelines for ...  -  21 Aug 2007
Medscape (subscription)Culture or polymerase chain reaction is recommended for testing antibiotic sensitivity but is not widely available. Culture is not as sensitive as rapid

Genes Identify Renal Graft Tolerant Patients  -  21 Aug 2007
MedPage Today,They then tested the genes with microarray and real-time polymerase chain reaction in samples from the other patients to determine whether they could

Asian Soybean Rust Found in Ghana for First Time  -  21 Aug 2007
Wisconsin Ag Connection,...and all 11 field samples amplified in a real-time fluorescent polymerase chain reaction (PCR) with P. pachyrhizi-specific primers.

Rampant Adoption of qRT-PCR and Next Generation DNA Sequencing ...  -  Aug 20, 2007
Business Wire (press release),...“Among the market segments, quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) technology, including instrumentation and reagents,

Thermus Aquaticus  -  Aug 17, 2007
Wyoming Tribune,...for the American company Cetus Corporation, invented a procedure that sped up the replication of DNA, a process named polymerase chain reaction, or PCR.

Effect of Human Papillomavirus 16/18 L1 Viruslike Particle Vaccine ...  -  Aug 14, 2007
Journal of American Medical Association (subscription),...probes and by polymerase chain reaction using SPF10 primers and a line probe assay detection system before vaccination and by polymerase chain reaction

Shareholders Q up to help raise funds  -  Aug 15, 2007
ic Wales,ReaX simplifies Polymerase Chain Reaction (PCR), a widely used laboratory technique for tasks such as blood testing, the diagnosis of diseases and the

Drug Maker Growth Without the FDA  -  Aug 14, 2007
Motley Fool...including the isolation of proteins and polymerase chain reaction (PCR) reagents and now has over 500 consumable products and automated systems.

EPA Demonstrates Faster Water Quality Test to Protect Beach Goers ...  -  Jul 26, 2007
U.S. EPA.gov (press release),By producing results in as little as three hours, the new method, quantitative Polymerase Chain Reaction (qPCR), will present public health officials with

Shrimp workshop nets admirers  -  Aug 16, 2007
Borneo Bulletin,She said she learnt actual real-time PCR (Polymerase Chain Reaction), and how to extract something and send it to the PCR lab. "Before this workshop, I knew

Dealing with hepatitis B and C in Punjab: Government hospitals don ...  -  Aug 3, 2007
Daily Times,By Muzaffar Ali LAHORE: Public hospitals in the city do not have Polymerase Chain Reaction (PCR) equipment to conduct quality, quantity and genotype tests

Diagnosis of Kingella kingae Osteoarticular Infection by PCR  -  Aug 1, 2007
AAP Grand Rounds (subscription),Specific real-time polymerase chain reaction places Kingella kingae as the most common cause of osteoarticular infections in young children.

Commentary: Considering Test Results in Context  -  Aug 16, 2007
TheHorse.com,Polymerase chain reaction (PCR) testing is an extremely sensitive test for biologic agents in horses. However, a positive PCR for Salmonella can indicate

Abbott Receives Supplemental FDA Approval for its Best-in-Class in ...  -  Aug 9, 2007
CNNMoney.comInitially approved in the United States on May 11, 2007, the RealTime test, based on real-time polymerase chain reaction (PCR) technology,

Sky News reporter  -  Aug 9, 2007
Sky News,The sample is extracted into liquid then subjected to a technique known as PCR (polymerase chain reaction) analysis. The process allows the DNA within the

Agilent expands microarray offering with FFPE sample labelling kit  -  Aug 16, 2007
DrugResearcher.com,...whole transcriptome qPCR (quantitative polymerase chain reaction) system to enable such experiments on Affymetrix' GeneChip 3' gene expression platform.

HPV-16/18 Vaccination Should Not Be Used to Treat Prevalent Infections  -  Aug 15, 2007
Medscape (subscription)...use of chemiluminescence with HPV RNA probes and by polymerase chain reaction (PCR) with use of SPF10 primers and a line probe assay detection system.

Biological Marker For Narcolepsy Identified  -  Aug 5, 2007
Science Daily (press release)The second screening of the samples was carried out by semiquantitative polymerase chain reaction (PCR) -- a biochemistry and molecular biology technique

Montana State envisions itself as University of the Yellowstone  -  Aug 15, 2007
Rocky Mountain News,His invention, the polymerase chain reaction technique, later sold for $300 million and in 1993 won Mullis the Nobel Prize for science.

Microbe magic: light to energy  -  Aug 4, 2007
Indian Express,T. aquaticus was crucial in making the polymerase chain reaction a routine procedure. PCR is used to amplify genetic material for testing and research.

Bird flu: Four Manipuri teens test negative  -  Aug 3, 2007
Times of India,...all under 14 years, from a farm in Chingmeirong village in Manipur was tested by RT-PCR (reverse transcriptase polymerase chain reaction).

Sickle Cell Foundation graduates 33 genetic counsellors  -  Aug 13, 2007
Vanguard,Foyinsola Oyebola, Oyagbola recalled that the first phase also brought about the provision of one Polymerase Chain Reaction (PCR) machine for prenatal

Cancer biomarker discredited  -  Aug 13, 2007
spectroscopyNOW.com (subscription),This was confirmed by Western blotting of the proteins from the same tissues and by reverse transcription polymerase chain reaction (RT-PCT) studies.

ND records first anthrax case of 2007  -  Jul 31, 2007
Bismarck Farm & Ranch Guide,A polymerase-chain-reaction (PCR) test confirmed the diagnosis of anthrax Friday. “Producers, especially those in areas that have recorded anthrax in the

An Overview of Pulmonary Diseases  -  Aug 13, 2007
Business Wire (press release),New technologies for infectious respiratory diagnosis: target-enriched multiplex polymerase chain reaction assays, multiplex reverse-transcriptase-PCR

Nasopharyngeal aspiration useful to confirm TB in children  -  Aug 13, 2007
Medicexchange (press release),Direct polymerase chain reaction analysis of nasopharyngeal aspirates was 62 per cent sensitive and 98 per cent specific in confirming culture-positive

Surprising New Species Of Light-harvesting Bacterium Discovered In ...  -  Jul 26, 2007
Science Daily (press release)Thermus aquaticus, which has revolutionized forensics and other fields by making the polymerase chain reaction (PCR) a routine procedure.

South Africa: Breakthrough in Study On Treating HIV Babies  -  Jul 26, 2007
AllAfrica.com,..."It may mean initiating treatment as soon as a diagnosis is made -- and that means SA has to get PCR (polymerase chain reaction) testing into every clinic,"

Instrument makers harness technology to pursue budding ...  -  Aug 13, 2007
Chemical & Engineering NewsIts BAX system adapts a polymerase chain reaction technique more closely associated with medical and biological research to test food for contaminants such

Mastodon genome sheds light on human evolution  -  Jul 23, 2007
New Scientist (subscription),Using a procedure called polymerase chain reaction (PCR) analysis, they next sequenced the entire mitochondrial genome of the mastodon, which stretches

Whirling disease changed how hatchery  -  Aug 12, 2007
Glenwood Springs Post Independent,The fish are raised and sent out for disease testing through a process called polymerase chain reaction (PCR), according to Capwell.

HIV Experts Want New Child TB Policy  -  Jul 23, 2007
Ontario Now,They require polymerase chain reaction tests which are expensive and not widely available. The problem is there is not ample resources to test everyone to

Industry news in brief - week 32  -  Aug 9, 2007
LabTechnologist.com,Fluidigm has appointed Bioke as its exclusive Benelux distributor for RT- PCR (real-time polymerase chain reaction) and genotyping products including the

Nutrition and brain function: Food for the  -  Aug 13, 2007
Checkbiotech.org (press release),Lau used real-time PCR (polymerase chain reaction) to find and measure expression of genes that produce iNOS and COX-2 in the stress-induced cell cultures.

WHO: Indonesia's withholding of viruses endangers world  -  Aug 7, 2007
CIDRAP,..."Although they were PCR [polymerase chain reaction] positive, they did not have living virus in them," he said. Indonesia is the only country that is not

HEALTH-INDIA: On War Footing to Stamp Out Bird Flu Outbreak  -  Aug 7, 2007
IPS,...and were quarantined have been declared negative for avian influenza virus by RT-PCR (Reverse Transcriptase Polymerase Chain Reaction) test," he said.

Comment on "Clinical Features of Bacterial Conjunctivitis in Children"  -  Jul 26, 2007
AEM (subscription)...testing for virus by cell culture with confirmatory immunofluorescense staining, polymerase chain reaction, or Rapid Pathogen Screening Adeno Detector.

MolecularMD Joins CML ALLIANCE(TM) to Provide Standardized qRT-PCR ...  -  Aug 6, 2007
Earthtimes.org...today announced its participation as a preferred provider of Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) patient monitoring

Culling tally crosses 3 lakhs  -  Aug 5, 2007
KanglaOnline,Delhi and found to be negative for avian influenza virus by RT-PCR (Reserved Transcriptase Polymerase Chain Reaction, the health official informed.

DNA-FluHunter - The all-rounder weapon against bird flu virus  -  Aug 3, 2007
openPR (press release),They are based on polymerase chain reaction technology. Such DNA are being used in reference laboratories like WHO laboratories for bird flu to reconfirm

Full Text  -  Aug 2, 2007
Science Magazine (subscription)Standard molecular techniques to trace tumor cells in blood, such as the polymerase chain reaction, don't allow this further analysis, says Claudio Orlando

Applied Biosystems breaks the $2bn barrier  -  Aug 2, 2007
LabTechnologist.com,The company's strong growth during the 2007 fiscal year was driven mainly by increased sales of Real-Time PCR (polymerase chain reaction) and applied

Fact sheet from the US Geological Research Center  -  Aug 1, 2007
Sawyer County Record,...important factors in how to isolate VHSV Genotype IVb using cell culture assays and its identification by the polymerase chain reaction (PCR) assay.

Study: Flu patients shed virus longer than expected  -  Aug 1, 2007
CIDRAP,Polymerase chain reaction (PCR) was used to test respiratory tract specimens for influenza A. If the initial test was positive, additional throat swabs were

GeneSearch™ BLN Approved for Detection of Breast Cancer Spread to ...  -  Aug 1, 2007
Cancer Consultants (press release),The GeneSearch BLN uses real-time reverse transcriptase-polymerase chain reaction (RT-PCR) to detect ammoglobin (MG) and cytokeratin 19 (CK 19) in lymph

BioTrove Reports Strong First Half  -  Aug 1, 2007
PharmaLive.com (press release),...multiple applications, including single nucleotide polymorphism (SNP) genotyping and real-time quantitative polymerase chain reaction (PCR)-based tests.

The measure of cell immortality  -  Jul 31, 2007
Chemistry World,...assay is currently the most widespread assay that does this, but it relies on the polymerase chain reaction (PCR) to amplify the DNA before detection.

K-State Veterinary Diagnostics Lab to debut new bovine health t  -  Jul 30, 2007
High Plains Journal,..."The polymerase-chain reaction test utilizes a proprietary process--a lysis buffer--that eliminates the need for the costly, labor-intensive and

Presence of lipoprotein lipase S447X stop codon affects the ...  -  Jul 30, 2007
The Journal of Thoracic and Cardiovascular SurgeryGenotyping for lipoprotein lipase S447X polymorphism was performed by polymerase chain reaction. Levels of interleukins 6 and 8 were measured before

I was just diagnosed with Lyme disease, but I heard the blood test ...  -  Jul 29, 2007
Scienceline,...(That being said, there is one test—polymerase chain reaction, or PCR—that can be used to identify the bacterium’s presence in your body by its DNA,

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